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Which of the following is NOT a natural means of DNA recombination?


A) Transformation in bacteria
B) Viral infection
C) PCR
D) Sexual reproduction

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How does DNA move during gel electrophoresis?


A) Larger pieces of DNA move at a faster rate.
B) DNA moves at a constant rate, regardless of fragment size.
C) The DNA moves toward the negative electrode.
D) Smaller pieces of DNA move faster toward the positive electrode.

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If you want to develop a procedure for identifying a genetic disorder caused by 20 different alleles, what method is most likely to be useful?


A) STRs
B) Arrays of DNA probes on filter paper
C) PCR
D) Gene therapy

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The enzymes used to cut genes in recombinant DNA research are called


A) spliceosomes.
B) DNA polymerases.
C) RNA polymerases.
D) replicases.
E) restriction enzymes.

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Describe one technique that can be used to generate multiple copies of a gene that is of interest to a researcher.

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The researcher can m...

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In recombinant DNA technology, plasmids


A) are isolated from the blood of mammals.
B) are the sites of protein synthesis in bacteria.
C) are used to insert foreign DNA into bacteria.
D) show restriction enzymes where to cut bacterial DNA.
E) are necessary for cellular respiration in certain plants.

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Controversy has arisen about using genetically engineered bovine growth hormone to increase milk yield in cattle. The lesson gained from this controversy is that


A) citizens should not be allowed to decide such issues, as they tend to make decisions based on emotion.
B) the whole human society should decide the proper uses of recombinant DNA technology.
C) society should let scientists decide the proper uses of recombinant DNA technology.
D) humans are not wise enough to use this technique properly.
E) recombinant DNA technology is inherently a bad idea and should be abandoned.

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Sequencing the genes of humans is important because


A) knowing the nucleotide sequence of human genes will aid in the treatment of diseases caused by them.
B) it will allow scientists to create human clones.
C) it will give us a better understanding of agricultural animals.
D) it provides information on how to build lipids in the cell.

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Stringent safety rules are not necessary for recombinant DNA research because it is a natural process that occurs in the absence of human intervention.

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The term transfecting refers to


A) making multiple copies of a small sample of DNA.
B) inserting the gene of interest into the host organism.
C) making GMOs that are sterile.
D) viral DNA becoming part of human DNA.

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All of the following are goals of biotechnology EXCEPT


A) treating disease more effectively.
B) improving agriculturally important food plants.
C) creating humans with higher intelligence levels.
D) generating economic benefits.
E) efficiently producing biologically important molecules.

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Sexual reproduction in humans is an example of DNA recombination.

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An important tool of genetic engineering is_________ DNA, which is DNA altered by the incorporation of genes from a different organism.

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A recombinant plasmid contains only human and bacterial DNA.

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Imagine that it is 1965 and the bacterium Thermus aquaticus has not yet been discovered. As a geneticist interested in forensics applications, you are attempting to develop a gene-amplifying technique similar to PCR. In a test tube, you place a DNA sample, free nucleotides, human DNA polymerase, and the appropriate primers for the DNA sequence being analyzed. You first heat the sample to 90°C, then cool it to 50°C, and then reheat it to 70°C. Based on this scenario and your knowledge of how modern PCR works, the result of these procedures will be


A) unsuccessful, because the enzyme will denature during the second step (cooling) .
B) successful copying of the DNA, because it follows modern PCR procedures almost perfectly.
C) unsuccessful, because the enzyme will denature during the third step (reheating) .
D) unsuccessful, because the enzyme will denature during the first step (heating) .
E) unsuccessful, because human DNA polymerase cannot recognize the primers used.

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_________are small, circular pieces of DNA that are often used in the process of transformation.

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Provide one example of a medical application of biotechnology.

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Possible answers are genetic s...

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Which of the following techniques amplifies a specific region of DNA?


A) DNA profiles
B) The use of DNA probes
C) Gel electrophoresis
D) PCR

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How is it possible for a dead bacterial cell to influence the DNA in a living bacterial cell?)

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Dead bacteria release plasmids...

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DNA recombination changes the genetic makeup of organisms.

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